Composite

Part:BBa_K136023:Design

Designed by: Kok-Phen YAN   Group: iGEM08_Paris   (2008-10-25)


strong promoter - RBS - lasR - double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

rbs-lasR-double terminator (backward insert) was cloned into the plasmid J61002 containing the promotor.


Source

Assembly of different parts from the registry.

References